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The Reyes Lab aims to understand the function of molecular machines as they work inside the cell. His group uses quantitative approaches in live cells, particularly through fluorescence microscopy, to infer the spatial organization macromolecules, the stoichiometry of molecular machines and the kinetics of cellular processes. Our current focus is in the understanding of the molecular machines of DNA replication. We use 鈥渟imple鈥� unicellular organisms, E. coli and S. cerevisiae, as model organisms for bacteria and eukaryotes.