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Zeiss Spinning Disk Confocal Microscope / TIRF

Zeiss Spinning Disk confocal microscope and TIRF
Image by Emily Pruner - 2020.

EQUIPMENT FEATURES SPECIFICATIONS

Stand model

Observer Z1

Features and Applications

Brightfield, DIC

4D Imaging (XY, Z-stack, Time-lapse)

Piezo Stage

Multi-Channel (up to 3 dyes simultaneous imaging)

FRET

FRAP

Live cell imaging.

Tiling.

Multipoint

Definite Focus

Piezo stage

TIRF

Inserts: Slides, culture cell plates and dishes

Light Sources

Laser lines: 405nm (50mW), 488nm (100mW), 561nm (75mW)

HXP 120V

Objectives

10x Plan Apo, DIC, NA 0.45

20x Plan Apo, DIC, NA 0.8

40x Plan Apo, DIC, NA 1.4 Oil

63x/ Alpha Plan Apo, NA 1.46, Oil, Corr, TIRF

Fluorescence Cubes

BP 450-490 / BP 500-550 (eGFP)

BP 532-558 / BP 570/640 (Cy3)

G 365 / BP 420-470 (DAPI)

TBP 483+564+642 / TBP 526+601+688 (GFP-mRFP+Alexa 633)

LSM Duo T20/R80 UV

TBP 405+488+561+640 nm / TBP 450+525+590+700 (DAPI, eGFP, Cy3, Alexa 633)

SD Emission filters

BP 450/50

BP 525/50

DBP 527/54+645/60

BP 629/62

BP690/50

Quad Emitter 446/523/600/677

Software

Zen

Detectors

Photometrics Prime 95B

Axiocam 506Mono

Location

MS2-094c

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