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GC-MS Systems

The Agilent 5975C Series GC/MSD with the Triple-Axis Detector GC-MS systems are used for targeted (SIM), untargeted and flux analysis experiments.听 Data are collected and analyzed by Chemstation software or Mass Hunter and Mass Profiler professional is used for untargeted differential analysis. Spectra are identified using Fiehn, Bains (Steadman Metabolism laboratory, Duke University) or NIST17 databases and authentic samples.

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LC-MS Systems

The Agilent 6470 Triple quadrupole LC-MS System听are designed to be compatible with ultra-high pressure liquid chromatography (UHPLC, 1290 Infinity LC System) separation for fast targeted analysis. The Multiple Reaction Monitoring strategy allows targeting of hundreds of compounds in a single run for accurate measurements. It is dedicated to low molecular-weight compounds and biomolecules analysis. We use the MassHunter software series for data acquisition, identification, and quantitation.

The Agilent 6540 UHD Accurate-Mass Q-TOF LC-MS system听is designed to be compatible with ultra-high pressure liquid chromatography (UHPLC, 1290 Infinity LC System) separation for fast and high confidence untargeted analysis of complex samples. It offers high dynamic range quantification as well as ultra-high-definition mass accuracy and mass resolution for MS and MS/MS data. We use the听Agilent Jet Stream听thermal-gradient-focusing technology to provide exceptional MS/MS sensitivity and identification of trace-level compounds.

Agilent 6545 UHD Accurate-Mass QTOF SFC/LC-MS system is designed to work either with super critical fluid chromatography or liquid chromatography.听 This is instrument, similar to the 6540, adds an orthogonal separation technique for the facility.

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Walk-up Media Analyzer

The BioProfile 400听analyzer is a user-friendly instrument that can be used to quickly听 (3 min/sample) measure glucose, lactate, glutamine, glutamate, ammonium, sodium and potassium concentrations as well as pH from tissue culture media or serum/plasma.听 For further information about this instrument and measurable concentration ranges, please visit the manufacturer鈥檚 website:

The BioProfile 400 is located on the 7th floor of the McIntyre building in room 726.听
To use the BioProvile 400, the researcher must first make an appointment with a core staff member for a training session that lasts about 30 minutes.
Sample requirements:
A minimum media volume of one milliliter is necessary for the proper use of this instrument.听 Media samples must be spun down to remove any cellular debris prior to analysis.

NMR

• QANUC 500 MHz NMR
• QANUC 800 MHz NMR

The staff of the MCF has access to the听QANUC NMR facility听and the MUHC-RI drug discovery platform systems which house 800MHz, 600MHz, 500MHz systems equiped with cryogenically cooled probes for improved sensitivity. 听

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Cellular Respiration - Seahorse Instruments

The facility has three seahorse instruments: 听XFe24 (24 well format), XFe96 (96 well format) and Xfp (8 well format). Using transient micro-chamber technology, the Seahorse instruments can measure the rate of oxygen consumption and the extracellular acidification rate of tissue culture cells (both adherent and suspension) using a specialized bicarbonate-free media system.听听 The cellular system can then be perturbed by the addition of nutrients, drugs or mitotoxins to measure changes in the rate of oxygen consumption (respiration) and the rate of acidification (glycolysis).听

The Seahorse instruments are located on the 7th floor of the McIntyre building in room 726.

Use of the Seahorse instruments requires approximately two days of training.听 The training will include instruction on how to calibrate the dosage of various mitotoxins (if desired) and general use of the instrument.听 An experienced trainer will be assigned to you.

Sample requirements:
Use of the Seahorse instruments does require tissue culture and growing cells using specialized media.听 If possible, tissue culture work should be done at the researcher鈥檚 site to prevent cross contamination in incubators & hoods.听 If this is not possible, other arrangements can be made.

Primary Contact: 听 听 听Dr. Daina Zofija Avizonis
听 听 听 听 听 听 听 听 听 听 听 听 听 听 听 听听Office: 418 tel: 514-398-5199
听 听 听 听 听 听 听 听 听 听 听 听 听 听 听 听 Lab: 401A听 tel: 514-398-2798
daina.avizonis [at] mcgill.ca (听听听听听听听听听听听听听听听听听听听听听听听听听听听听听听听听听)